Type: HPLC Application #: 108210
Detection: UV, 230nm Conditions: eluent A: 0.1% TFA in water, eluent B: 80% acetonitrile in water + 0.1% TFA, conditioning about 10min with eluent A, after injection linear gradient from 0 to 40% B in 12min, from 40 to 65% B in 58min, 5min at 65% B, then in 5min from 65 to 0% B flow rate 0.7ml/min pressure 50bar temperature 40°C Authors: Macherey-Nagel Source: LC department
Details
Legend: HPLC of a hydrophilic standard protein mixture: 1. cytochrome c (MW 12500), 2.
lysozyme (14000), 3. trypsinogen (24000), 4. trypsininhibitor soybean (21500),
5. chymotrypsinogen (25000), 6. carboanh